There are two possible starting points for mutanalysts.
One is proving a sequence and the mutations sampled, for which the mutational load, mutational spectrum and the mutational bias indicators will be calculated.
The other is more downstream, wherein one proves a mutational spectrum and mutational load and the mutational bias indicators will be calculated.
If you want to know what mutations you have in a series of ab1 files check
out out Mutantcaller.
If you want to know the library composition (e.g. redundancy) check out PedelAA or go to the bottom of this page.
In frame sequence that was mutagenised. Note that all symbols that aren't uppecase ATUGC, will be discarded along with a Fasta header (e.g. '>T. maritima Cystathionine β-lyase'), therefore for masked sequences use lowercase.
This is the list of the mutations found. Identifying the mutations can be done using the Mutantcaller tool.
The average is N/A mutations per sequence (N/A kb).
The sample variance is N/A mutations per sequence.
The λPoisson is N/A mutations per sequence.
Rows represent the wildtype base, while columns the base in the mutant.
|Data display options||Raw data||Frequency normalised||Strand complimentary normalised|
Sequence-composition–corrected incidence of mutations (%):
|A→N, T→N (%)|
|Transitions (%) total|
|A→G, T→C (%)|
|G→A, C→T (%)|
|transversions (%) Total|
|A→T, T→A (%)|
|A→C, T→G (%)|
|G→C, C→G (%)|
|G→T, C→A (%)|
For details about pedel-AA see pedel-AA homepage.