MutanalystMutanalyst logo

Mutational load and spectrum calculator for epPCR libraries with poor sampling

Choose starting point

There are two possible starting points for mutanalysts.
One is proving a sequence and the mutations sampled, for which the mutational load, mutational spectrum and the mutational bias indicators will be calculated.
The other is more downstream, wherein one proves a mutational spectrum and mutational load and the mutational bias indicators will be calculated.

Choose:

If you want to know what mutations you have in a series of ab1 files check out out Mutantcaller.
If you want to know the library composition (e.g. redundancy) check out PedelAA or go to the bottom of this page.

Starting from a sequence and a mutant genotype list

Sequence

In frame sequence that was mutagenised. Note that all symbols that aren't uppecase ATUGC, will be discarded along with a Fasta header (e.g. '>T. maritima Cystathionine β-lyase'), therefore for masked sequences use lowercase.

Sequence


Library size

For Pedel-AA calculations, the library size is required.
Size

Mutations found

This is the list of the mutations found. Identifying the mutations can be done using the Mutantcaller tool.

Variants

Mutational frequency

The average is N/A mutations per sequence (N/A kb).

The sample variance is N/A mutations per sequence.

The λPoisson is N/A mutations per sequence.


Starting from a table of tallied nucleotide specific mutations  

Rows represent the wildtype base, while columns the base in the mutant.

Load
Save
 Previous
To
From  
A T G C
A
T
G
C



Proportion of adenine
%
Proportion of thymine
%
Proportion of guanine
%
Proportion of cytosine
%

Corrected mutation incidence

Data display options Raw data Frequency normalised Strand complimentary normalised

Sequence-composition–corrected incidence of mutations (%):

From/To A T G C
A
T
G
C

Graphical Representation

A G C T

Download

Bias indicators

Indicator Calculated Estimated error
Ts/Tv
AT→GC/GC→AT
A→N, T→N (%)
G→N,C→N (%)
AT→GC (%)
GC→AT (%)
Transitions (%) total
A→G, T→C (%)
G→A, C→T (%)
transversions (%) Total
A→T, T→A (%)
A→C, T→G (%)
G→C, C→G (%)
G→T, C→A (%)


Pedel-AA results

For details about pedel-AA see pedel-AA homepage.